Fig. 14. Effect of VEGF on luteal cells to determine its effect on TSP system in vitro luteal cell culture. Briefly, isolated cells of CL were stimulated with increasing concentrations of VEGF (0, 1, 10, and 100 ng/ ml) at 75-80% confluency over a period of 24, 48 and 72 h (n = 3/condition). Cells lysate was used for the extraction of total RNA and cDNA preparation. Two-way ANOVA with Tukey's HSD was done to find the effect of VEGF concentration and time on the mRNA expression of TSP and its receptor system. Each point in the line chart indicates Mean ± SEM. The different superscripts denotes statistical significance (p<0.05). Abbreviations: TSP1, Thrombospondin1; mRNA, Messenger RNA; TSP, Thrombospondin; CD, cluster of differentiation.